GELRITE®

Productnummer: 71010-52-1 268,10

gelling agent for microbiological culture media
Alternative to Agar

CAS-Nr. [71010-52-1]
EG-Nr. 275-117-5

GELRITE® is a highly-purified, natural heteropolysaccharide which forms stable, agar-like gels when exposed to soluble salts. Compared to agar however, only about half the amount is required.

GELRITE® has been specially developed as a gelling agent for microbiological culture media. It can however, replace agar in other applications, too.

Polymerisation of GELRITE® is enhanced in the presence of (bivalent) kations. It is therefore supplemented by MgCl2 if a solid gel structure is required. Elevated salt concentrations increase gelling temperature (e.g. 500 mM Na+ to approx. 68 °C with 1 % GELRITE®). When using very high salt concentrations like e.g. seawater, gelling capacity may be significantly reduced.

Chemical composition:

Linear polysaccharide comprising of O-acetylate glycuronic acid, glucose
and rhamnose units.

Advantages of GELRITE® compared to Agar:

  • consistent product quality
  • almost 100 % batch-to-batch conformity
  • forms remarkably clear gels
  • reduces preparation time as gels form faster
  • remains stable even at high temperatures and is therefore also ideal for thermophilic microorganisms; gels can withstand repeated autoclaving without any changes to gel strength
  • indicates no toxic contaminations 
    (e.g. through phenols, as found in agar)
  • is chemically inert against most media additives
  • is resistant to enzymatic degradation
  • is non-toxic (no signs of toxicity in rats exposed to a dose 
    of 5.000 mg/kg)
  • is very economical to use

 

Optie Verp Verpakking
1 500 g plastic
2 1 kg plastic